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The Enzymatic Reduction of Hydroxyurea to Urea by Mouse Liver

Laboratory of Molecular Pharmacology, National Cancer Institute, NIH, Bethesda, Maryland 20014

The conversion of hydroxyurea to urea by mouse liver whole homogenate and cellular subfractions has been studied. Urea-¹⁴C was separated from the hydroxyurea by means of paper electrophoresis and determined quantitatively by liquid scintillation spectrophotometry. The results of these studies show that the reaction is an enzymatic reduction utilizing reduced pyridine nucleotides and is stimulated by flavin adenine dinucleotide. Cell fractionation studies indicate that the liver mitochondria carry out a substantial portion of this conversion.

¹ Present address: Division of Oncology, The Johns Hopkins University School of Medicine, Baltimore, Md. 21205.

Received 5/ 7/69. Accepted 1/23/70.

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