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Characterization of a Rapidly Growing AKR Lymphoblastic Cell Line Maintaining Gross Antigens and Viral Replication

Virus and Disease Modification Section [W. A. W., J. G. M., M. A. C.], and Ultrastructural Studies Section [N. A. W.], Viral Leukemia and Lymphoma Branch, Etiology, National Cancer Institute, Bethesda, Maryland 20014

A continuous line of lymphoblastic cells (AKR-A) was established in tissue culture from a spontaneous transplantable tumor of AKR mice. The cells have the following characteristics: (a) rapid growth in suspension, (b) lack of adhesion to surfaces, (c) viral and cellular antigens of the Gross leukemia virus complex, (d) shedding of infectious Gross leukemia virus, and (e) tumorigenicity in AKR mice. Electron microscopy of early passages revealed large macrophages actively engulfing nonviable AKR-A cells. Clumps of viable AKR-A cells were adherent to the macrophage surface, presenting a false picture of emperipolesis.

Received 12/23/69. Accepted 4/ 8/70.