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Delayed Hypersensitivity and Neoplasia: in Vitro Studies with Macrophage Migration¹

Charleston Foundation Cancer Research Laboratory, West Virginia University School of Medicine, Morgantown, West Virginia 26506

With the use of in vitro macrophage migration for demonstrating delayed hypersensitivity, the reactions of peritoneal cells from tumor-bearing and normal animals were studied in extracts of homologous tumor, normal tissue, and specific antigen. Rats sensitized to tuberculin evidenced inhibition of cell migration when cultured in media containing purified protein derivative, and injection of 1.0, 10.0, or 20.0 million tumor cells s.c. 10 days prior to culture had no effect on this inhibition. Both normal rats and mice and those bearing transplantable tumors showed enhanced cell migrations when cultured in the presence of either normal tissue or pooled homologous tumor extracts. One group of rats were given injections of 1.0 x 10⁶ tumor cells in the hindfoot, and after 10 days these legs were surgically removed. At that time or 2 weeks later, in vitro macrophage migrations were examined in the presence of extracts of the rat's own tumor, pooled tumor, and normal tissue. The rats showed enhanced cell migrations in extracts of normal tissue or pooled tumor. In extracts of the rat's tumor only, there was a tendency toward inhibition of cell migration. Results are considered along with other investigations conducted in this field. The mechanics of this in vitro system of delayed hypersensitivity are discussed.

¹ This work was supported by USPHS Grant 8631-13-434. Presented in part at the 59th Annual Meeting of the American Association for Cancer Research, April 1968, Atlantic City, N. J.

² Present address: Department of Pathology, Mayo Graduate School of Medicine, University of Minnesota, Rochester, Minn. 55901.

Received 1/22/70. Accepted 5/ 7/70.