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Proliferative Kinetics of Human Hematopoietic Cells during Different Growth Phases in Vitro¹

Divisions of Chemotherapy Research [A. T., A. S., B. D. C.] and Biophysics [J. F.], Sloan-Kettering Institute for Cancer Research and Cornell University Medical College, New York, New York 10021

The proliferation kinetics of an established human hematopoietic cell line (SK-L7) derived from the peripheral blood cells of a patient with acute myeloblastic leukemia were studied during different phases of cell growth with thymidine-³H and autoradiographic methods. As the population changes from logarithmic to stationary growth, the S, G₂, and G₁ phases of the cell cycle become longer and more variable; G₁ is most affected, and an increased proportion of cells remain in G₁. Both nutrient deficiency and the extent of cell confluence affect the cell cycle as the cultures approach saturation density; the changes are reversible if the cells are fed and their population density is reduced. The kinetic behavior of SK-L7 cells in many ways resembles that of leukemic cells in the marrow in acute leukemia, and this system may be a useful in vitro model to study growth-regulatory factors and chemotherapeutic effects.

¹ This investigation was supported in part by USPHS Research Grants CA-05826 and CA-08748 from the National Cancer Institute, the American Cancer Society Grant T-538A, the John C. McCarroll Fund, Hearst Foundation Fund, United Leukemia Fund, and the Junior Committee of the Society of Memorial Sloan-Kettering Cancer Center.

² Presently with Tenri Hospital, Nara-Prefecture, Japan.

³ Leukemia Society of America Special Fellow.

Received 8/20/70. Accepted 5/13/71.