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The Anionic Nature of Sarcoma 180 Cell Surfaces, and Sensitivity to 4,4'-Diacetyldiphenylurea-bis(guanylhydrazone)¹

Departments of Experimental Pathology and Experimental Therapeutics, Roswell Park Memorial Institute, Buffalo, New York 14203

The electrokinetic surfaces of sublines of murine Sarcoma 180 cells, which were resistant or sensitive to 4,4'-diacetyldiphenylurea-bis(guanylhydrazone)-dimethane sulfonate (DDUG) were examined. No significant differences were detected between either the net negative surface charges of the two cell lines or the respective contributions to cell surface negativity of bound, anionic groups susceptible to neuraminidase or RNase. However, the alteration of the response of the two sublines to growth inhibition by DDUG after RNase and neuraminidase treatments was significantly different. Treatments with these enzymes increased the resistance of the sensitive cells to DDUG 3- and 2-fold, respectively, while the response of the Sarcoma 180 DDUG-resistant subline to the drug was unaltered by enzyme treatment. The findings are discussed with regard to the structure and charge characteristics of the DDUG molecule in relation to the possible differences in the spatial arrangements of the anionic sites at the surfaces of the sensitive and resistant cells.

¹ Supported in part by USPHS Grants CA-04175 and CA-11047 from the National Cancer Institute and by June Lambert Memorial Grant P403-D from the American Cancer Society, Inc.

Received 3/ 1/71. Accepted 5/17/71.