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Irreversible Change of the Pattern of Carcinogenic Aminoazo Dye-binding Proteins in Rat Liver during Continuous Feeding of 3'-Methyl-4-dimethylaminoazobenzene¹

Zoological Institute, Faculty of Science, University of Tokyo, Tokyo, Japan

A single large dose (40 mg) of 3'-methyl-4-dimethylaminoazobenzene was given to groups of rats with different histories of previous dye feeding: no, 2 weeks, or 1 month of dye feeding followed by 2 weeks or 1 month of basal diet feeding. The pattern of dye-binding proteins in the heat-treated (55°, 3 min) cell sap of the liver was analyzed by chromatography on carboxymethylcellulose as well as by gel filtration in Sephadex G-100.

Dye-binding protein patterns of rats which had been fed on the dye-containing diet for 2 weeks or 1 month in the past differed from those of rats with no such history of dye feeding and resembled those of rats killed immediately after continuous dye feeding. The results seem to suggest that the changing pattern of dye-binding proteins in the course of continuous dye feeding is irreversible. Particular emphasis was placed on dye binding to the nonbasic proteins instead of to the more basic proteins with regard to the possible involvement of dye-protein binding in the mechanism of carcinogenesis.

¹ This is Paper 3 of the series, of "Studies on Carcinogen-binding Proteins" which has been supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Japan.

Received 2/24/71. Accepted 6/ 3/71.