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Cartesian Diver Studies on Respiration and Glycolysis of High and Low Sarcoma Lines

Department of Biology, Graduate School of Arts and Science, New York University, New York, New York 10003

Two cell types grown in tissue culture, designated high line (NCTC 2472) and low line (NCTC 2555), were studied by Cartesian diver manometric techniques. Studies with NaOH and NaHCO₃ as absorbant seals in stoppered microdivers suggest that consistency of respiration of both cell lines may be regulated by the type of seal used in the neck of the divers. Sodium lauroyl sarcosine, at 0.0018 M, inhibits high-line respiration by 65%, and low-line respiration, by only 10%. Both cell lines appear to be almost equally sensitive to 3-nitropropionic acid and to urethan. The most outstanding difference between the two cell lines was their anaerobic glycolysis, of which the low line was approximately one–third that of the high-line cells. Glycolysis in the high-line cells is significantly inhibited by iodoacetic acid and sodium lauroyl sarcosine, whereas sodium lauroyl sarcosine does not significantly influence low-line cells. L-Asparaginase demonstrates no inhibitory effect. It is suggested that both cell lines may have equally functional citric acid cycles and cytochrome systems. The main difference appears to be the more direct dependence of high-line cells for glucose in both glycolytic and respiratory process.

¹ This study was supported by a Damon Runyon Cancer Research Traineeship.

Received 2/22/71. Accepted 7/ 1/71.