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Comparative Oligonucleotide Sequences of 28 S Ribosomal RNA of Normal Liver and Novikoff Hepatoma Ascites Cells¹

Department of Pharmacology, Baylor College of Medicine, Houston, Texas 77025

The distributions and structures of complete pancreatic RNase digestion products of 28 S rRNA were compared for the Novikoff hepatoma and rat liver. The oligonucleotides of the digests were separated on diethylaminoethyl Sephadex A-25 at pH 7.6 according to chain length, and the mono-, di-, octa-, nona-, and decanucleotides were compared separately after isolation of the single components by paper electrophoresis at pH 3.5 (mono- and dinucleotides) or by rechromatography on diethylaminoethyl Sephadex A-25 at pH 3.3 (octa-, nona-, and decanucleotides). On the basis of a calculated chain length of 4500 for 28 S rRNA, the frequencies of the mono- and dinucleotides were defined; they differed mainly in the dinucleotide G-Up, which occurs 134 and 103 times in 28 S rRNA of the Novikoff hepatoma and rat liver, respectively. The polypurine sequences G-A-G-G-A-A-G-Cp, A-A-A-A-G-G-A-A-Cp, A-G-A-G-G-A-A-A-Cp, and G-G-G-A-A-G-A-G-Up, and the partial sequences of a number of other octa-, nona-, and decanucleotides were found in both the 28 S rRNA of rat liver and of the Novikoff hepatoma. They were identified by their positions in the elution profiles of the subfractionations on diethylaminoethyl Sephadex A-25 at pH 3.3 and by their compositions as determined after complete digestion with T₁ RNase.

¹ These studies were supported by Cancer Research Center Grant CA-10893, P-1, and American Cancer Society Grant P-339.

² Present address: Innere Klinik und Poliklinik, Tumorforschung, Ruhruniversität, D-4300 Essen, Hufelandstrasse 55, West Germany.

Received 5/20/71. Accepted 7/14/71.