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Differences in the Immunogenicity of Leukemia L1210 Sublines in DBA/2 Mice¹

Department of Experimental Therapeutics, Roswell Park Memorial Institute, New York State Department of Health, Buffalo, New York 14203

After the i.p. inoculation of 10³, 10⁴, or 10⁵ leukemia L1210 cells, the survival of DBA/2Ha-DD mice was longer than that of DBA/2J or DBA/2Cr mice. This difference was greatly reduced in mice that received total body X-radiation (250 R) the day prior to cell inoculation and in nonirradiated mice after the inoculation of 10⁶ or 10⁷ L1210 cells. DBA/2Ha-DD, DBA/2J, and DBA/2Cr mice survived longer after the inoculation of cells from an L1210 line resistant to methylglyoxal-bis(guanylhydrazone) than after inoculation of cells from the parent L1210 line. These differences were also reduced in preirradiated mice.

Treatments with low doses of arabinosylcytosine were more effective against the L1210 cells resistant to methylglyoxal-bis(guanylhydrazone) than against the parent line in both DBA/2Ha-DD and DBA/2J mice; similar differences in therapeutic response were seen in DBA/2Ha-DD mice treated with 4,4'-diacetyldiphenylurea-bis(guanylhydrazone), 2-chloro-4',4''-di-2-imidazolin-2-ylterephthalanilide, or carzinostatin but were not seen in those treated with methotrexate. In DBA/2Ha-DD mice, arabinosylcytosine was also more effective against an L1210 line resistant to 4,4'-diacetyldiphenylurea-bis(guanylhydrazone) than against the parent line. All of these therapeutic differences were greatly reduced or abolished in preirradiated mice.

On the basis of transplantation tests in DBA/2Ha-DD and DBA/2J mice, a line of L1210 grown in culture for several months appeared to be more immunogenic than were any of the in vivo lines of L1210 examined.

¹ This investigation was supported in part by Research Grants CA-10805 and CA-11047 from the National Cancer Institute, USPHS.

Received 6/17/71. Accepted 8/13/71.