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Isolation of Enzymatically Active Nuclei from Epithelial Cells of the Trachea

Chemistry Branch and Lung Cancer Unit, National Cancer Institute, NIH, Bethesda, Maryland 20014

A simple method for the isolation of nuclei from tracheal epithelial cells is described. The isolated nuclei are morphologically well preserved and can be suspended in reproducible aliquots. RNA polymerase and exoribonuclease activity can be measured in the isolated nuclei.

Received 7/14/70. Accepted 11/13/70.