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A Human Leukocyte Culture with an Unusual Cytoplasmic Envelopment of Herpes-type Particles¹

Department of Viral Biology, The John L. Smith Memorial Institute for Cancer Research, Pfizer Inc., Maywood, New Jersey 07607 [S. C., W. K. E. M.]; Roosevelt Hospital, New York, New York 10019 [R. P. A.]; and Bionetics Research Laboratories, Bethesda, Maryland 20014 [D. P. A. F.]

A leukocyte culture, G-6, was established from a pretreatment sample of peripheral blood from a 33-year-old patient with chronic myelogenous leukemia. When studied under time-lapse cinematography, G-6 cells exhibited great surface activity, not observed in other cultured leukocyte cells. A majority of G-6 cells had 45 chromosomes. Herpes-type particles in this culture acquired their envelopes in the cytoplasm of infected cells in a manner not previously reported in any of the established human leukocyte cultures.

¹ This study was conducted under Contracts NIH 70-2080 and NIH 67-661 within the Special Virus Leukemia Program of the National Cancer Institute, NIH, USPHS.

² Recipient of a grant from Whitehall Foundation, East Hampton, New York.

Received 8/24/70. Accepted 12/14/70.