This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Stulberg, M. P. Articles by Shugart, L. R. Search for Related Content PubMed PubMed Citation Articles by Stulberg, M. P. Articles by Shugart, L. R.

Response of Modified Phenylalanine Transfer RNA to Recognition by Phenylalanyl Transfer RNA Synthetase¹

Biology Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37830

Undermethylated Escherichia coli tRNA^Phe has reduced activity in aminoacylation when measured by differences in initial velocities; however, the K_m remains unchanged. The conformation of undermethylated tRNA^Phe, measured by optical methods, is different from its normal counterpart. The ability of undermethylated tRNA^Phe to support poly U-stimulated polyphenylalanine synthesis is 3-fold less than the normal molecule.

¹ Research jointly sponsored by the National Institute of General Medical Sciences, NIH, and the U. S. Atomic Energy Commission under contract with Union Carbide Corporation.