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The Adoptive Transfer of Concomitant Immunity to Murine Tumor Isografts with Spleen Cells from Tumor-bearing Animals

Surgery Branch, National Cancer Institute, NIH, Bethesda, Maryland 20014 [P. J. D., Y. H. P.]; Emory University, School of Medicine, Atlanta, Georgia 30322 [B. W. E.]; and Duke University, School of Medicine, Durham, North Carolina 27706 [B. S.T.]

A methylcholanthrene-induced fibrosarcoma (MCA-10) in male and female C57BL/6 mice was used to study the presence of concomitant immunity in a syngeneic murine tumor-host system. A group of 1125 mice was inoculated s.c. with 10⁵ viable MCA-10 cells in the right hind limb. Groups of 25 mice randomly selected from the original 1125 mice were challenged with 5 x 10³ MCA-10 cells s.c. in the left hind limb 1, 7, 14, 21, and 28 days after initial tumor transplantation. Untreated mice received 5 x 10³ MCA-10 cells at identical times. At 7, 14, 21, 28, and 35 days after initial tumor transplantation, four additional groups of 50 MCA-10 tumor-bearing mice were sacrificed, the tumor weight to total body weight ratio was calculated, and a spleen cell suspension was prepared. Viable spleen cells from these animals (5 x 10⁷ to 5 x 10⁸) were injected i.p., into four groups of 25 untreated syngeneic mice. MCA-10 cells (5 x 10³) were inoculated s.c. into mice in each group 1, 7, 14, or 21 days after spleen cell transfer. Controls received tumor cells alone, or tumor cells and spleen cells from normal syngeneic mice.

In the groups of tumor-bearing mice, the challenge tumor cell inocula grew in 0 to 8% of animals at all intervals after initial tumor transplantation except Day 1. Tumors developed in 87 to 100% of control animals. At day 1, tumors developed similarly in the left hind limb of both control and tumor-bearing animals. Mice which received spleen cells from tumor-bearing animals manifested a significant delay in tumor development and/or decrease in tumor incidence at all initial tumor transplantation-spleen cell transfer time intervals except 1 and 7 days.

Received 11/16/70. Accepted 1/27/71.

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