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Ferritin-labeled Antibody Studies of Feline C-type Particles¹

Viral and Rickettsial Disease Laboratory, California State Department of Public Health, Berkeley, California 94704 [L. S. O., J. L. R., D. O. N. T., E. H. L.]; and Viral Carcinogenesis Branch, National Cancer Institute, NIH, Bethesda, Maryland 20014 [R. J. H.]

The application of an indirect ferritin-labeled antibody method to tissue culture lines established from cats suffering from various malignant diseases revealed tagging of viruses and of certain areas of the cell surface membranes. Dog antifeline sarcoma and cat antifeline sarcoma sera were used as the intermediate sera on feline C-type virus-positive lines established from 3 cases of malignant lymphoma, 1 erythroleukemia, and 1 idiopathic agranulocytosis. The tagging of viruses and membranes was similar in all the lines, indicating a similarity or sharing of antigenic components. Each instance of ferritin-labeled antibody tagging previously had been shown to be positive with fluorescent antibody staining, showing that there is correlation between fluorescent antibody staining of feline C-type virus-producing cells and ferritin tagging of the virus and infection-mediated membrane antigens.

¹ This study was supported by Contract PH 43-68-997 from the National Cancer Institute and Grant AI 08200 from the National Institutes of Allergy and Infectious Diseases, NIH, USPHS, Department of Health, Education, and Welfare.

Received 1/25/71. Accepted 4/ 7/71.