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The Developmental, Noduligenic, and Tumorigenic Potentials of Transplanted Mammary Glands and Primary Ducts from C3H Mice Previously Fed a Phenylalanine-deficient Diet¹

Cancer Research Genetics Laboratory [Y. H. H., K. B. D.] and the Department of Nutritional Sciences [Y. H. H., G. M. B.], University of California, Berkeley, California 94720

This paper reports the developmental, noduligenic, and tumorigenic capabilities of transplanted mammary glands and primary ducts from C3H/Crgl mice previously fed a phenylalanine-deficient diet. The donor mice had been previously fed various levels of phenylalanine, and one-half of them had been stimulated with pituitary isografts. All were 24 to 25 weeks old, had been on the dietary regimens since they were 5 weeks old, and had not developed nodules. The host mice were virgin female C3H/Crgl mice. Each host mouse received a primary duct transplant in each of its gland-free inguinal fat pads, a whole-gland transplant, and a pituitary isograft under the left kidney capsule. After 12 weeks of stimulation, the pituitary isograft was destroyed. Five weeks later, the mice were sacrificed, and the host glands and transplants were examined for the presence of nodules and tumors. Nodules were present in the mammary glands of all of the host mice, in all of the normal outgrowths from the primary duct transplants, and in all but two of the whole-gland transplants. The transplanted mammary glands and primary duct outgrowths were well developed and resembled the host glands. Mammary tumors occurred in 14% of the transplants but in none of the host glands. These results show that the developmental, the noduligenic, and tumorigenic capabilities of the donor mammary glands were not permanently impaired by a phenylalanine-deficient diet.

¹ This investigation was supported in part by USPHS Research Grant CA 05388 from the National Cancer Institute.

Received 8/12/71. Accepted 8/26/71.