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Suppression of SV40 T Antigen in Transformed Hamster Cells by Chronic Treatment with 5-Bromo-2-deoxyuridine¹

Departments of Pathology [J. W. K., S. H. S.] and Biological Chemistry [E. A. D.], College of Medicine, Milton S. Hershey Medical Center, The Pennsylvania State University, Hershey, Pennsylvania 17033, and The Wistar Institute [B. D. V.], Philadelphia, Pennsylvania 19146

5-Bromo-2-deoxyuridine, a thymidine analog, can suppress phenotypic expression. It can also suppress T antigen, a nuclear protein that is characteristic of SV40-transformed cells. T antigen suppression was demonstrated by the use of both complement fixation and immunofluorescence. Cellular proliferation was not significantly affected at the lowest doses that suppressed the T antigen; however, higher doses inhibited proliferation. Suppression of T antigen was a relatively selective phenomenon, since cells treated with the higher doses enlarged and accumulated DNA, RNA, protein, acid phosphatase, and cytochrome oxidase. DNA and protein synthesis continued even at the highest 5-bromo-2-deoxyuridine dose levels.

¹ Supported by USPHS Grants 1-R01-CA11097, 5T01-CA05163, and AM-12074.

² Recipient of Career Development Award 7-K4-CA38809.

³ Present address: Department of Experimental Pathology, Scripps Clinic and Research Foundation, La Jolla, Calif. 92037.

Received 3/17/72. Accepted 6/19/72.