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Regulated Transport of Ribosomal Subunits from Regenerating Rat Liver Nuclei in a Cell-free System¹

Department of Physiological Chemistry, Ohio State University College of Medicine, Columbus, Ohio 43210

A cell-free system is described which permits the study of the control of ribosomal RNA processing in and transport from isolated rat liver nuclei. The transport of the RNA in the form of 60 S and 40 S ribosomal subunits was energy and temperature dependent. It was also dependent on the presence of macromolecules in the cytosol but was independent of ongoing RNA synthesis.

¹ This work was supported by Grant CA 12411-01 from the National Cancer Institute, NIH, Bethesda, Md.

² To whom requests for reprints should be sent.

Received 5/ 2/72. Accepted 7/24/72.