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A Comparison of Polypyrimidine Fragments in 28 S Ribosomal RNA from Rat Liver and the Novikoff Ascites Hepatoma¹

Tumor By-Products Laboratory, Department of Pharmacology, Baylor College of Medicine, Houston, Texas 77025

The distributions of polypyrimidine sequences have been compared for ribosomal 28 S RNA from the Novikoff hepatoma and normal rat liver. Complete digestion at adenylyl and guanylyl residues by successive treatment with U2 RNase followed by T₁ RNase released oligonucleotides from highly labeled ribosomal RNA. These oligonucleotides were isolated by chromatography according to chain length on diethyl-aminoethyl Sephadex A-25 at pH 7.6 followed by electrophoresis on cellulose acetate or Whatman No. 3MM paper at pH 3.5. Approximately 60 oligonucleotides with chain lengths of 6 to 17 and with the same compositions and electrophoretic mobilities were isolated individually or in simple mixtures from 28 S ribosomal RNA of normal liver and the Novikoff hepatoma. The data obtained provide further support for the conclusion that large portions of 28 S ribosomal RNA from normal liver and the Novikoff hepatoma have very similar nucleotide sequences.

¹ These studies were supported in part by USPHS Grant CA-10893 and American Cancer Society Grant NP-53G.

Received 6/ 6/72. Accepted 7/24/72.