This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Strzinek, R. A. Articles by Norton, S. J. Search for Related Content PubMed PubMed Citation Articles by Strzinek, R. A. Articles by Norton, S. J.

The Purification and Characterization of Liver Glyoxalase I from Normal Mice and from Mice Bearing a Lymphosarcoma¹

Departments of Biological Sciences [R. A. S., V. E. S.] and Chemistry [S. J. N.], North Texas State University, Denton, Texas 76203

Glyoxalase I has been purified from the livers of normal DBA/1J mice (565-fold) and mice bearing a lymphosarcoma (348-fold). There was a significant difference in the fractionation patterns of the glyoxalase I activities from the two sources. Molecular weight determinations for which Sephadex gel filtration was used were conducted on the purified enzymes. The estimated molecular weights are 48,000 for the normal liver enzyme and 60,000 for the "tumor liver" enzyme. Treatment with guanidine-HCl has no effect on the molecular weight of liver glyoxalase I from normal mice; however, similar treatment of the liver enzyme from tumor-bearing mice decreases the molecular weight of catalytically active enzyme to approximately 23,000. The K_m values of the enzymes are similar. Studies with the tumor (lymphosarcoma) enzyme indicate that the liver enzyme from tumor-bearing mice is not of tumor origin. The results of the investigation suggest that the glyoxalase I enzyme in the liver of mice bearing a lymphosarcoma is modified due to the presence of the tumor.

¹ This work was supported in part by Grants B-133 and B-268 from The Robert A. Welch Foundation of Texas and Ca07527 from the NIH.

² This work has been submitted in partial fulfillment of the requirements of the Ph.D. degree. Present address: Life Sciences Directorate, Cellular Analytical Section, DB/22, NASA-MSC, Houston, Texas 77058.

Received 7/20/70. Accepted 7/24/72.