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Factors Related to Therapeutic Efficacy in Adoptive Chemoimmunotherapy of a Friend Virus-induced Lymphoma¹

Division of Oncology, Department of Medicine, University of Washington School of Medicine, Seattle, Washington 98195

Adult C57BL/6 mice given syngeneic Friend lymphoma cells (FBL-3) usually died on Day 12 to 15. Treatment on Day 5 with cyclophosphamide (CY), 180 mg/kg, and lymphoid cells from C57BL/6 mice immunized with FBL-3 cured most mice, whereas cells alone had no effect and CY alone or with nonimmune cells only doubled the median survival time. As an adjunct to CY, at least 3 x 10⁶ spleen cells or peripheral blood lymphocytes from mice immune to FBL-3 were needed for cure to occur. Spleen cells from mice immunized with FBL-3 three times were somewhat more effective than cells from mice immunized once or eight times. Treatment with CY plus spleen cells from mice immunized with a BALB/c Moloney lymphoma or with Moloney sarcoma virus-immunogens sharing antigens with FBL-3—was highly effective. Thus, lymphoid cells had to be immune to tumor-specific cellular and/or virion antigens to be effective in this chemoimmuno therapy model. Finally, long-term survivors of chemoimmunotherapy resisted challenge with FBL-3 cells and their spleen cells were in turn effective in the chemoimmunotherapy model.

¹ This work was supported by Grant CA 10777 and CA 05231 from the National Cancer Institute, NIH, USPHS, and the Institutional Cancer Grant IN-26 from the American Cancer Society.

² Special Fellow of the Leukemia Society of America.

³ Scholar of the Leukemia Society of America.

Received 5/ 8/72. Accepted 8/ 1/72.