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Biochemical and Cytogenetic Characterization of Rat Hepatoma Cell Lines in Vitro¹

Departments of Oral Biology [C. D.], Biochemistry [E. J. M.], and Pediatrics [M. M. C.], Schools of Dentistry and Medicine, State University of New York at Buffalo, Buffalo, New York 14226, and Division of Human Genetics, Children's Hospital of Buffalo, Buffalo, New York 14222

Comparative studies on H4-II-E-C3 cells, originating from a rat hepatoma, and two lines derived therefrom are described. On the basis of morphology, kinetics of growth, tumorigenicity, and karyotype, the three lines segregated into two distinct groups. All three cell lines were, however, related as evidenced by the presence of certain marker chromosomes. Isozyme measurements could be used to separate these cells into three distinct groups. Quantitative biochemical differences were also discerned among all three lines. These were based on the inducibility of tyrosine aminotransferase with steroid and the activity profile of pyridine-adenine dinucleotide transhydrogenase during the growth cycle.

These studies illustrate the necessity for use of multiple parameters to characterize evolving cell lines in vitro. Furthermore, they suggest the feasibility of using a combination of cytogenetic and biochemical measurements, in conjunction with model systems, for the study of mammalian cell genetics and the question of tumorigenesis.

¹ A preliminary report was presented at the 22nd Annual Meeting of the Tissue Culture Association, Lake Placid, New York, June 1971.

² Supported by Grant P-513A from the American Cancer Society.

³ Supported by Grant FD-ES 00466-01 MCHB from the Food and Drug Administration, HEW.

⁴ Supported by Grant HD 05187 from the NIH and Project No. 417 from the Department of Maternal and Child Health, HEW. Present address: Department of Human Genetics, Hadassah-Hebrew University Medical Center, Jerusalem, Israel.

Received 1/12/72. Accepted 8/ 2/72.