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Coating of Friend Leukemia Virus after Treatment with Specific Antiserum¹

Division of Cytology [T. S., E. de H.], Division of Immunology [C. S.], Sloan-Kettering Institute for Cancer Research, New York, New York 10021, and The Center for Experimental Cell Biology, Mollie B. Roth Laboratory, the Mount Sinai School of Medicine of the City University of New York, New York, New York 10029 [C. F.]

After Friend leukemia cells were treated with specific antiserum to Friend leukemia virus (FLV) both in vitro and in Millipore diffusion chambers in vivo in FLV-immunized mice, extracellular and budding viruses were coated with flocculent or filamentous material of variable thickness. Coats observed on viruses after 3 weeks in chamber cultures in FLV-immunized mice were denser and thicker than those seen after in vitro incubation with FLV antiserum. Almost all "enveloped A" as well as type C viruses were coated after FLV antiserum treatment in the two experimental systems. Virus budding sites were also occasionally coated, while the rest of the cell surface was not.

As compared with untreated cultures, the number of budding viruses increased significantly in all cell samples grown in diffusion chambers, particularly in those taken from FLV-immunized mice.

Ferritin or southern bean mosaic virus, used in conjunction with hybrid antibodies to detect virus-bound -globulin, labeled more than 90% of viruses treated with FLV antiserum in vitro, in contrast to 9% in controls treated with normal mouse sera; this strongly suggests the presence of FLV antibody within the coat.

¹ Supported in part by National Cancer Institute Grants CA-10,000, CA-13,047, and CA-08748; by the Health Research Council of the City of New York Grant I-325; and a grant from the John A. Hartford Foundation, Inc.

Received 7/14/72. Accepted 8/29/72.