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Experimental Infection of Human Cervix by Herpesvirus Type 2 in Organ Culture¹

Departments of Microbiology and of Obstetrics and Gynecology, The University of Rochester School of Medicine and Dentistry, Rochester, New York 14642

A simple experimental procedure for the study of herpesvirus infection in organ cultures of human cervical tissues is discussed. The use of this procedure has allowed us to conclude that the columnar epithelium and mucous glands of the endocervix, squamous epithelium of the ectocervix, and stromal tissues in both endo- and ectocervix all are susceptible to herpesvirus type 2. Infection spreads more rapidly in stromal tissue than in the epithelial tissues of endo- and ectocervix. Cells with intranuclear inclusions and with "ground-glass" nuclei occur in the same organ culture but in different proportions, depending on the type of tissue and the time after infection.

¹ This work was aided by USPHS Research Grant CA-05206 from the National Cancer Institute, Grant IN-18L from the American Cancer Society, and by a grant from the Labor Foundation.

Received 8/12/71. Accepted 10/ 7/71.