This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Janss, D. H. Articles by Irving, C. C. Search for Related Content PubMed PubMed Citation Articles by Janss, D. H. Articles by Irving, C. C.

The Binding of 7,12-Dimethylbenz(a)anthracene to Mammary Parenchyma DNA and Protein in Vivo¹

Veterans Administration Hospital, Memphis 38104 [D. H. J., C. C. I.], and Department of Physiology and Biophysics [D. H. J., R. C. M.], and Department of Biochemistry [D. H. J., C. C. I.], University of Tennessee, Memphis, Tennessee 38103

Sprague-Dawley female rats were sacrificed at various times after the intragastric administration of 7,12-dimethylbenz(a)anthracene (DMBA)-³H. The abdominal-inguinal mammary glands were removed, and the parenchymal cells were separated. Parenchymal cell DNA and protein were isolated, and the DNA was purified by density equilibrium centrifugation in cesium chloride. Sixteen hr after carcinogen feeding, DMBA binding amounted to 47 pmoles/mg of DNA. Fifty % of the DMBA bound to DNA at 16 hr was present 14 days after administration, and 31% was still detectable at 42 days. The amount of DMBA binding to protein was less than one-half that observed bound to DNA. Furthermore, by 14 days, DMBA binding to protein had declined to just detectable levels. Increased parenchymal cell DNA content, due to cellular proliferation resulting from DMBA-induced tumorigenesis, was not responsible for the decreased DNA specific activity observed at 14 and 42 days. Thus, persistent binding of DMBA to rat mammary parenchymal cell DNA in vivo was demonstrated.

¹ Supported by the United states Veterans Administration and by USPHS Grants CA 05490 and CA 12630 from the National Cancer Institute.

Received 7/26/71. Accepted 10/ 7/71.

This article has been cited by other articles:

				T. L. Weimer, A. P. Reddy, U. Harttig, D. Alexander, S. C. Stamm, M. R. Miller, W. Baird, J. Hendricks, and G. Bailey Influence of {beta}-Naphthoflavone on 7,12-Dimethylbenz(a)anthracene Metabolism, DNA Adduction, and Tumorigenicity in Rainbow Trout Toxicol. Sci., October 1, 2000; 57(2): 217 - 228. [Abstract] [Full Text] [PDF]

				A. Izzotti, A. Camoirano, C. Cartiglia, C. J. Grubbs, R. A. Lubet, G. J. Kelloff, and S. D. Flora Patterns of DNA Adduct Formation in Liver and Mammary Epithelial Cells of Rats Treated with 7,12-Dimethylbenz(a)anthracene, and Selective Effects of Chemopreventive Agents Cancer Res., September 1, 1999; 59(17): 4285 - 4290. [Abstract] [Full Text] [PDF]