This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Shearer, R. W. Articles by Smuckler, E. A. Search for Related Content PubMed PubMed Citation Articles by Shearer, R. W. Articles by Smuckler, E. A.

Altered Regulation of the Transport of RNA from Nucleus to Cytoplasm in Rat Hepatoma Cells¹

Department of Pathology, School of Medicine, University of Washington, Seattle, Washington 98195

RNA base sequences that are restricted to the nucleus in normal rat liver are found in the cytoplasm in azo dye-induced primary hepatomas. Some of these RNA's are present in the cytoplasm of 19-day fetal liver and of liver regenerating 2.5 hr after 30% hepatectomy.

No RNA's were found to be restricted to the nucleus in the tumor cells, suggesting that the mechanism of selective transport of RNA between nucleus and cytoplasm may be lost during malignant transformation. This migration of RNA out of the tumor cell nucleus is an early effect of the carcinogen, appearing in a significant fraction of the liver cells after the animals were fed a diet containing 0.06% 3'-methyl-4-dimethylaminoazobenzene for only 6 days. Liver cells of rats fed the noncarcinogenic analog aminoazobenzene have nucleus-restricted RNA equivalent to that of normal liver.

¹ Supported by USPHS Grants CA-42423, CA-10188, and AM-08686; American Cancer Society Grant E-480A; and an institutional grant from the American Cancer Society.

² Present address: Fred Hutchinson Cancer Research Center, Pacific Northwest Research Foundation, 1102 Columbia Street, Seattle, Wash. 98104.

Received 6/28/71. Accepted 10/26/71.

This article has been cited by other articles:

				D Gillespie and R. Gallo RNA processing and RNA tumor virus origin and evolution Science, May 23, 1975; 188(4190): 802 - 811. [Abstract] [PDF]