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Effects of Cytosine Arabinoside and 1,3-Bis(2-chloroethyl)-1-nitrosourea on Human Glial Tumor Cells¹

Division of Neurological Surgery, Indiana University Medical Center, Indianapolis, Indiana 46202

The effects of 1-ß-D-arabinofuranosylcytosine were examined in cultures of 5 glial tumor cell strains derived from different patients. The growth inhibition by 1-ß-D-arabinofuranosylcytosine was assessed by counting the number of cells remaining in microtest plates. A significant drug effect was demonstrated against all 5 cell strains. Inhibition was dependent on dosage and duration of treatment. When doses reached the level of 0.5 g/ml, however, there were no further increases in inhibitory response. The degree of recovery from growth inhibition likewise became less marked with higher dosage and longer treatment. The surviving cells remained in situ without further proliferation. The cytotoxic effect was shown to be enhanced by combination treatment with 1,3-bis(2-chloroethyl)-1-nitrosourea (8.0 µg/ml).

¹ Supported by USPHS Grant CA-06145 from the National Cancer Institute.

Received 3/24/71. Accepted 11/19/71.