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The Effect of cis-Platinum (II) diamminodichloride on Bone Marrow¹

Miroslav ák, Jaroslav Drobník² and Zdenk Ren

Biophysics Department, Faculty of General Medicine, Charles University, Prague, Czechoslovakia [M. Z., Z. R.], and Institute of Macromolecular Chemistry, Czechoslovak Academy of Sciences, Prague 6-Petiny 1888, Czechoslovakia [J. D.]

Changes in bone marrow elements after a single i.p. injection of cis-platinum (II) diamminodichloride, 8 mg/kg, were analyzed in mice. Cell counts in unit volumes of bone marrow, mitotic indices, and relative frequencies of individual elements were estimated 1, 3, 6, and 24 hr and 3, 6, and 10 days after drug administration. It was found that changes in mitotic index occurred prior to changes in cell count. Mitotic index and the cell count decreased from Hr 1 reaching minimum values after 24 and 72 hr, respectively. Normal values were then reached after oscillations. Only small changes were observed in erythroblasts, whereas more mature normoblasts decreased on Day 3 and later increased. With myeloid cells, pronounced changes occurred in the number of more mature forms. Oscillations of decreasing amplitude, observed in lymphocytes, monocytes, and megakaryocytes, were characterized by a high rise early after administration followed by a decrease below control values and, finally, a trend toward increase to normal values. Most probably, the slight oscillations extended longer than the 10-day period and will be studied later. We concluded that the observed changes after a dose of 8 mg/kg corresponded to, for example, rather low doses of X-irradiation.

¹ This work was partially supported by a research contract from Engelhard Minerals and Chemical Corp. to the Biophysic Department of Michigan State University.

² To whom correspondence should be addressed.

Received 6/16/71. Accepted 12/ 8/71.