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The Binding of 9,10-Dimethyl-1,2-benzanthracene to Mouse Epidermal Satellite DNA in Vivo

Chemistry Branch, Etiology, National Cancer Institute, NIH, Bethesda, Maryland 20014

We measured the relative binding of the labeled carcinogen 9,10-dimethyl-1,2-benzanthracene-³H (DMBA-³H) to mouse satellite and main-band epidermal DNA 24 hr after the topical application of the carcinogen to mouse skin. Labeled DMBA-bound DNA was fractionated into satellite and main-band components by composite gel electrophoresis and by isopycnic CsCl centrifugation. With both fractionation methods, extensive binding of DMBA-³H to mouse satellite DNA was observed. The amount of DMBA-³H bound per mg of DNA was the same for both satellite and main-band DNA.

Received 10/ 4/71. Accepted 12/ 9/71.