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Tumor-specific Resistance in Mice Detected by Inhibition of Macrophage Migration¹

Section of Experimental Radiotherapy [J. V., R. D. J.], and Department of Biomathematics [B. W. B.], University of Texas at Houston, M. D. Anderson Hospital and Tumor Institute, Houston, Texas 77025

Resistance against a methylcholanthrene-induced fibrosarcoma and a spontaneous mammary carcinoma in syngeneic C3Hf mice was demonstrated in vitro by specific inhibition by tumor antigens of peritoneal cell emigration from capillary tubes. The soluble antigens of the two tumors did not cross-react, but strong nonspecific inhibition of migration was observed when tumor cells or particulate matter was present in the reaction mixture.

The degree of inhibition of macrophage migration was related to the stage of the development of antitumor immunity in the peritoneal cell donor. Depression of fully developed immunity by excess tumor antigen (desensitization) was not reflected in the motility of the peritoneal cells of the host in the presence of tumor antigen.

Host resistance could also be detected by a simple but nonquantitative procedure in which the effect of tumor antigens on macrophage spreading was observed in ordinary culture dish or microtest plate cultures of peritoneal exudate cells.

¹ This work was supported in part by USPHS Grants CA05047, CA6294, CA11138, and CA11430.

² Present address: Department of Radiation Medicine, Massachusetts General Hospital, Boston, Mass. 02114.

³ Doctoral candidate at The University of Texas at Houston Graduate School of Biomedical Sciences, Houston, Texas 77025.

Received 7/ 1/71. Accepted 12/28/71.