This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Baird, W. M. Articles by Boutwell, R. K. Search for Related Content PubMed PubMed Citation Articles by Baird, W. M. Articles by Boutwell, R. K.

Acrylamide Gel Electrophoresis Studies of the Incorporation of Cytidine-³H into Mouse Skin RNA at Early Times after Treatment with Phorbol Esters¹

McArdle Laboratory for Cancer Research, University of Wisconsin Medical Center, Madison, Wisconsin 53706

The tumor-promoting activities of multiple applications of phorbol and of four phorbol diesters as well as the effect of a single application of each on the incorporation of cytidine-³H into skin RNA were determined in female Charles River CD1 mice. Effective tumor formation correlated with a stimulation of cytidine-³H incorporation.

The nature of the early RNA response was analyzed by polyacrylamide gel electrophoresis. The extraction procedure allowed quantitative isolation of undegraded RNA from whole mouse skin. By 3 hr after treatment with effective tumor promoters, there was a large stimulation of incorporation of cytidine-³H into "32 S" RNA, rRNA, and 4 to 5 S RNA. Stimulation of cytidine-³H incorporation into 32 S RNA was observed as early as 30 min after treatment with the most effective promoter, 12-O-tetradecanoyl-phorbol-13-acetate. Comparison of the responses in cytidine-³H incorporation 3 hr after treatment with tumor promoting phorbol esters and 2 irritants suggested that the phorbol esters were able to induce increased incorporation into 32 S RNA earlier than the irritants, while both caused increased incorporation into the 4 to 5 S RNA.

¹ This work was supported in part by grants from the American Cancer Society (E-6M) and the National Cancer Institute (CA-07175 and CA-05002).

Received 8/23/71. Accepted 12/29/71.