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Lymphocyte Blastogenic Responses to Cultured Allogeneic Tumor Cells in Vitro¹

Departments of Developmental Therapeutics [R. J. A., E. M. H.] and Surgery [C. M. M.], The University of Texas at Houston, M. D. Anderson Hospital and Tumor Institute, Texas Medical Center, Houston, Texas 77025 [R. J. A., E. M. H.]

For development and evaluation of systems through which tumor immunity might be evaluated in man, allogeneic tumor cell-lymphocyte interactions were studied with the one-way mixed culture technique. The critical importance of dose-response studies in the evaluation of this interaction was demonstrated. Maximal blastogenic lymphocyte responses were induced by 5 x 10⁴ irradiated tumor cells, and inhibition of blastogenesis was induced by 10⁶ tumor cells. The median maximal blastogenic responses produced by the tumor cells was 3,700 cpm, compared to that produced by phytohemagglutinin (58,000 cpm), streptolysin O (17,400 cpm), streptokinase-streptodornase (13,500 cpm), allogeneic lymphocytes (30,500 cpm), or allogeneic irradiated lymphoblasts (51,000 cpm). The inhibition of blastogenesis produced by higher doses of tumor cells was caused by a nondialyzable factor released into the supernatant of cultured, irradiated, or mitomycin-treated cells. This study indicates that inhibitors influence lymphocyte blastogenic responses to tumor cells in vitro and suggests that such factors may influence host response to tumors.

¹ This research was supported by Grant ACS CI 22 of the American Cancer Society and Grants CA 05931 and CA 11520 from the National Cancer Institute, USPHS, Bethesda, Md. 20014.

Received 10/12/71. Accepted 2/11/72.