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Synchronization of Cell Division in Vivo through the Combined Use of Cytosine Arabinoside and Colcemid¹

Departments of Pathology, University of Pittsburgh Schools of Medicine and Dental Medicine, Pittsburgh, Pennsylvania 15213 [R. S. V., G. D.], and Fels Research Institute, Temple University, School of Medicine, Philadelphia, Pennsylvania 19140 [H. L., E. F.]

Mitotic activity in Lieberkühn's crypts increased from the 3 to 5% levels normally occurring in 0.9% NaCl solution-injected rats to peak values of 15% in animals treated with a single injection of either ara-C or Colcemid. In animals treated with ara-C followed by Colcemid, 33 to 38% of the crypt epithelial components could be identified as dividing cells, thus indicating a synchronization of at least two-thirds of the proliferating epithelial cells. Of additional significance was the finding that these regimens were not necrogenic to the intestinal epithelium. Further studies utilizing a second injection of Colcemid indicated that synchrony was lost once traversal through the cell cycle was reinstituted.

¹ This research was supported in part by Grants CA-11390 and CA-12218 from the National Cancer Institute, Grant AM-14882 from the National Institute of Arthritis and Metabolic Diseases, and Grant BC-7N from the American Cancer Society.

² Recipient of a Career Development Award (5KO4-DE35155) from the National Institute of Dental Research.

Received 12/22/71. Accepted 3/29/72.