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Metabolism and Biological Effects of N⁶-(²-Isopentenyl)-adenosine on a Cell Line Derived from Circulating Leukocytes of a Patient with Chronic Myelogenous Leukemia¹

Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada

N⁶-(²-Isopentenyl)adenosine (i⁶ Ado) at a concentration of 3 µM inhibits the growth of a line of cells derived from the circulating leukocytes of a patient with myelogenous leukemia, Roswell Park Memorial Institute line 6410. No alteration in the alkaline phosphatase activity of these cells is observed under these conditions.

i⁶ Ado is a component of 6410 cell tRNA but could not be detected as a nucleoside free in the cells or culture medium. The tRNA of cells cultured in the presence of i⁶ Ado contains a normal complement of ²-isopentenyl groups.

Line 6410 cells contain an enzyme activity that converts i⁶Ado to the corresponding base, which is much less toxic to these cells.

¹ This work was supported by a grant from the National Cancer Institute of Canada and by Grant MT-2738 from the Medical Research Council of Canada.

² Research Fellow of the National Cancer Institute of Canada.

Received 10/26/71. Accepted 4/18/72.