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Antigenic Alteration of Lymphoblastic Leukemic Cells Induced by Cell Culture and Hydrocortisone¹

Departments of Radiology [G. M. K.], Immunology and Microbiology [W. A. C.], and Biochemistry and Molecular Biology [R. C.], University of Oklahoma School of Medicine, and Cancer [G. M. K.] and Biochemistry [R. C.] Sections, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma 73104; and Phillips University, Enid, Oklahoma 73701 [J. C. B.]

Cytotoxic, complement-dependent antibodies in antisera from goats immunized with lymphoblastic leukemic cells show immunological properties that differ, depending on how the cells used for immunization were grown. Cytotoxic antibodies in antiserum to cells grown in medium with added hydrocortisone could not be completely absorbed by cells grown in the mouse peritoneal cavity (in vivo) or grown in vitro without added hydrocortisone. Similarly, in vivo cells failed to absorb completely cytotoxic antibodies in antiserum to cells grown in vitro without added hydrocortisone. However, cells grown in vitro absorbed completely antibody to in vivo cells. In each case, all cytotoxic antibodies were removed by absorption with homologous cells.

The amount of Vibrio cholerae neuraminidase-cleavable, cell-surface sialic acid was greatest in cells grown in vivo and least in cells cultured in medium with added hydrocortisone. Treatment of in vivo cells with Vibrio cholerae neuraminidase to remove cell-surface sialic acid did not affect their capacity to absorb cytotoxic antibodies from either homologous or heterologous antisera.

¹ This investigation was supported in part by NIH Grant NS09176 and Research Cancer Development Award 5-K3-AM-38649.

² Former Fleming Scholar, Cancer Section, Oklahoma Medical Research Foundation, Oklahoma City, Okla. 73104.

Received 2/15/72. Accepted 6/ 1/72.