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The Effect of Cytosine Arabinoside on Virus Production in Various Cells Infected with Herpes Simplex Virus Types 1 and 2¹

Department of Microbiology, College of Medicine, The Milton S. Hershey Medical Center of The Pennsylvania State University, Hershey, Pennsylvania 17033

Experiments on the inhibition of the replication of herpes simplex virus types 1 and 2 in human embryonic kidney and primary rabbit kidney cells by cytosine arabinoside have shown that synthesis of infectious herpes simplex virus type 1 is 2 to 3 times more sensitive to the analog than that of type 2. With labeled analog, it appears that the relatively greater concentrations of cytosine arabinoside necessary in hamster embryo fibroblast cells, compared with rabbit cells, is probably due to the less efficient uptake of the analog by these cells. Isopycnic cesium chloride centrifugations of Sarkosyl-Pronase digests of cells have shown that cytosine arabinoside may be incorporated into herpesvirus DNA as well as into cellular DNA, the uptake into the DNA of herpes simplex virus type 2 being somewhat greater than that of type 1.

¹ This study was conducted, in part, under Contract 70-2024 within the Special Virus Cancer Program of the National Cancer Institute, NIH, USPHS, and Grant PS-59 from the American Cancer Society.

Received 8/18/72. Accepted 10/11/72.