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Proliferation Kinetics of Density-inhibited Cultures of Human Cells, A Complex in Vitro Cell System¹

Department of Physiology, Harvard University School of Public Health, Boston, Massachusetts 02115

Proliferation kinetics and cell cycle parameters have been measured in exponentially growing and density-inhibited (daily medium changes) stable-plateau phase cultures of Chang human liver (LICH) cells. The mean duration of all phases of the cell cycle among the cycling population was approximately doubled in density-inhibited cultures. About 50% of the population was effectively noncycling but still viable. The lower rate of proliferation was balanced by cell loss into the culture medium leading to a constant cell number and a cell turnover rate of 20% per day. Density-inhibited cultures of LICH cells possess a number of the kinetic characteristics of tumors in vivo and may provide a useful in vitro system for a study of the effects of some chemotherapeutic agents, protracted schedules of X-irradiation, and of interaction between the two.

¹ Supported by Research Grants CA-11751 and EH-00002 from the NIH.

² Recipient of Special Fellowship CA-45249 from the National Cancer Institute. Present address: Deptartment of Radiation Therapy and Nuclear Medicine, Thomas Jefferson University Hospital, Philadelphia, Pa. 19107.

Received 2/19/73. Accepted 6/ 8/73.

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