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Incorporation of 2-Deoxy-D-glucose into Glycoproteins of Normal and Simian Virus 40-transformed Hamster Cells¹

Department of Virology and Epidemiology, Baylor College of Medicine, Houston, Texas 77025

2-Deoxy-D-glucose, which was regarded as being poorly metabolized by animal cells and therefore useful for transport studies, is incorporated rapidly and intact into glycoprotein. The polyacrylamide gel profile of the incorporation of 2-deoxy-D-glucose into glycoprotein parallels that of glucosamine and fucose. The incorporation of 2-deoxy-D-glucose into glycoprotein and glycolipid may help to explain several of the inhibitory features of 2-deoxy-D-glucose on virus-mediated events. Profiles of the glycoproteins obtained by polyacrylamide gel electrophoresis reveal that the simian virus 40-transformed cells have a reduced amount of a high-molecular-weight glycoprotein as compared to normal cells.

¹ This investigation was supported in part by Research Contract NO 1 CP 33257 within the Virus Cancer Program of the National Cancer Institute, NIH, Bethesda, Md., and by Research Grant ACS-IN-27M from the American Cancer Society.

² Recipient of Special Fellowship Award 1-F3-CA54,999 from the National Cancer Institute, NIH.

Received 10/ 9/72. Accepted 6/18/73.

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