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Competitive DNA-RNA Hybridization of Nuclear and Microsomal RNA in Normal, Neoplastic, and Neonatal Liver Tissue¹

Departments of Oncology and Pathology and the McArdle Laboratory for Cancer Research, University of Wisconsin Medical School, Madison, Wisconsin 53706

The nuclear and microsomal RNA of three minimal deviation hepatomas and 10-day-old neonatal rat liver were compared by standard DNA-RNA competitive hybridization techniques. nRNA from 5123C hepatoma and 10-day neonatal liver lack sequences present in adult liver nuclear RNA. These missing sequences appear to be identical in part. nRNA from the slowly growing Morris 9618 hepatoma could not be distinguished from adult liver nRNA. The microsomal RNA from this tumor and the 7800 hepatoma both showed sequences to be missing which were present in adult liver microsomal RNA, whereas microsomal RNA from the Morris 5123C hepatoma could not be distinguished from adult liver microsomal RNA in relation to their competitive efficiency for liver nuclear RNA. The significance of these findings in relation to transport of mRNA from nucleus to cytoplasm and in cytoplasmic mRNA stabilization is considered.

¹ This work was supported in part by grants from the National Cancer Institute (CA-07175) and the American Cancer Society (E-588). Portions of the work reported were taken from a thesis submitted by C. T. G. in partial fulfillment of the requirements for the PhD. degree in the Graduate School of the University of Wisconsin.

² Postdoctoral trainee in Biochemical Pathology of the National Institute of General Medical Sciences (GM-00130). Present address: Department of Pathology, School of Medicine, University of South Florida, Tampa, Fla.

³ Predoctoral trainee of the National Cancer Institute (T01-CA-05002).

Received 1/ 5/73. Accepted 7/ 2/73.