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Blastogenic Response of Lymphoid Cells to Tumor Cells or Tumor Membrane Extracts in Vitro¹

Department of Pathology, Monash University, Melbourne, Australia

The in vitro cellular response of mouse spleen cells to tumor cells or tumor membrane extract was compared using the one-way mixed culture technique. The maximal primary response of spleen cells to mitomycin C-treated Ehrlich ascites carcinoma cells in vitro occurred on the 3rd to 5th days when the spleen cells to tumor ratio was 5:1. Spleen cells from immune animals responded earlier and to a greater degree than spleen cells from normal animals. Membrane extract also stimulated spleen cells from normal animals similarly to mitomycin C-treated tumor cells but the response of spleen cells to membrane extract appeared to be limited to an antigen concentration between 0.01 and 1 µg/ml. Reduction of thymidine-³H uptake by spleen cells occurred in the presence of high concentration of tumor cells or membrane extract, indicating that these may interfere with blast transformation of lymphoid cells in vitro.

¹ This work was supported by grants from the Anti-Cancer Council of Victoria and the Australian Research Grants Committee.

² To whom requests for reprints should be addressed at Department of Pathology, Monash University Medical School, Prahran, Victoria, Australia, 3181.

Received 5/10/73. Accepted 7/18/73.