This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Ogawa, M. Articles by McCulloch, E. A. Search for Related Content PubMed PubMed Citation Articles by Ogawa, M. Articles by McCulloch, E. A.

Studies of Nitrogen Mustard Transport by Mouse Myeloma and Hemopoietic Precursor Cells¹

Department of Medicine, The Ontario Cancer Institute, and the University of Toronto, Toronto, Ontario, Canada

We studied the transport of nitrogen mustard (HN2) by mouse hemopoietic and transplantable myeloma (Adj. PC-5 and MOPC 460D) stem cells, using gradients of temperature and choline chloride. Bone marrow and myeloma cells were exposed to HN2 in culture at various temperatures, washed, and tested for colony-forming efficiency. The protective effect of lowered temperatures was similar for normal marrow colony-forming units and both lines of myeloma cells. When cells were exposed to HN2 in the presence of choline chloride, the survival of colony-forming units increased, and the dose response curves were similar among the three classes of cells. We were unable to demonstrate a qualitative difference in the transport of HN2 by mouse hemopoietic precursor cells and the stem cells of two myelomas with different sensitivities to HN2.

¹ This investigation was supported by a grant from The Ontario Cancer Treatment and Research Foundation.

² Research Fellow of The Ontario Cancer Treatment and Research Foundation.

Received 6/ 8/73. Accepted 9/ 5/73.