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Effect of Arginine on the Incorporation of ³²P into Rat Liver and Novikoff Hepatoma Cytosol Proteins¹

Institut du Cancer Montréal, Hôpital Notre-Dame, and Département de Biochimie, Université de Montréal, Montréal, Canada

It was observed that rat liver cytosol can transfer ³²P from ³²PO₄ to acceptor proteins. ³²P-labeled proteins co-purify with a class of proteins known to accept arginine at their NH₂-terminal end. When arginine-¹⁴C is added to the incubation mixture, ³²P incorporation is stimulated and small molecules bearing both ³²P and ¹⁴C labels can be released by treating labeled proteins with leucine aminopeptidase. Novikoff hepatoma seems to have lost the ability of phosphate fixation, even though it can incorporate arginine.

¹ This work was supported by grants from the National Cancer Institute of Canada and also by grants from La Fondation J. H. Biermans and les Fondations J. Rhéaume.

² Fellow of the National Cancer Institute of Canada. Present address: Hôpital Honoré Mercier, St. Hyacinthe, Qué, Canada.

³ Research Associate of the National Cancer Institute of Canada.

⁴ Monat Scholar of the Université de Montréal.

Received 7/13/73. Accepted 9/11/73.