This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Eaton, M. D. Articles by Scala, A. R. Search for Related Content PubMed PubMed Citation Articles by Eaton, M. D. Articles by Scala, A. R.

Enhancement of Lymphoma Cell Immunogenicity by Infection with Nononcogenic Virus¹

Departments of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115 [A. R. S.], and Medical Microbiology, Stanford University School of Medicine, Stanford, California 94305 [J. A. H.]

For preparation of antigen, cells from an ascites lymphoma, induced by the Gross virus and transplanted in syngeneic C3H/Bi mice, were infected with parainfluenza I or Newcastle disease virus. After 48 hr of incubation to permit growth of virus, cells were extracted by freezing and thawing in 0.03% NaCl solution. Extracts representing about 10⁷ tumor cells were injected repeatedly at intervals of 3 to 4 weeks. Survival after i.p. challenge with uninfected lymphoma cells was demonstrated. Immunized allogeneic C3H/HeJ mice survived larger challenge doses than did the syngeneic C3H/Bi strain. Control mice of either strain given injections of extracts of uninfected lymphoma cells did not develop significant immunity. The two viral species seemed equally effective in enhancing immunogenicity. Preliminary results indicate that growth of virus in the tumor cells may be essential to immunogenicity of these preparations. Complete Freund's adjuvant improved the immune response by making it possible to induce immunity with fewer injections of virus-cell antigen. Tests of cell fractions in syngeneic mice indicate that a major portion of the antigen is in the membrane fraction, less is in other fractions, and none is in the 20,000 x g supernatant. Virus and other small particles concentrated from the tissue culture supernatants by ultracentrifugation were less effective than membranes.

¹ This research was aided by a grant from the Leukemia Society of America and by NIH General Research Support Grant to Harvard Medical School.

² Present address: Department of Medical Microbiology, Stanford University School of Medicine, Stanford, Calif. 94305.

³ Present address: Department of Pathology, Harvard Medical School, Boston, Mass. 02115.

Received 6/25/73. Accepted 9/10/73.