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Transplantability and Biological Behavior of Mouse Liver Tumors Induced by Ethylnitrosourea¹

Departments of Pathology [A. P. K.] and Radiology [S. D. V.], The Pritzker School of Medicine and Argonne Cancer Research Hospital [S. D. V.] The University of Chicago,^,4, Chicago, Illinois 60637

A study has been made of the transplantability, morphology, and biological behavior of hepatomas induced by ethylnitrosourea in C57BL x C3H F₁ newborn and adult mice. Eight tumors induced in newborn and five in adult mice were transplanted to isogeneic hosts, and their behavior has been followed for a period of 1 year.

Grafts from the neonatally induced hepatomas grew in 75% of the recipients and were serially transplanted for 22 generations. Transplantation time was reduced from the original 8.4 weeks to 2 weeks. These transplants grew expansively, invaded locally, and metastasized to regional and distant lymph nodes and the lungs, killing the recipients within 6 weeks.

Hepatomas that originated in mice treated with ethylnitrosourea as adults grew in 30% of the recipients, but growth was slower. The original transplantation time of 18.0 weeks has been reduced to 8.1 weeks during the observed five transplant generations. No metastascs were observed thus far in this series.

¹ This investigation has been supported by NIH-NCI-69-2087 and USPHS 5-P06-RR-00409-04.

² Present address: Department of Pathology, Diagnostic and Therapeutic Institute of Piraeus and Cancer Research Center, 51 Botassi Street, Piraeus, Greece.

³ To whom requests for reprints should be addressed.

⁴ Operated by the University of Chicago for the United States Atomic Energy Commission.

Received 7/13/72. Accepted 10/27/72.