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Radioiron Incorporation by a Transplantable Lymphoid Tumor¹

Department of Veterinary Pathology and the Institute of Comparative Medicine, College of Veterinary Medicine, University of Georgia, Athens, Georgia 30601

The in vivo uptake of iron by tissues in chickens inoculated with a transplantable lymphoid tumor was studied, and ⁵⁹Fe-labeled transferrin was used as a tracer. The transplantable lymphoid tumor incorporated ⁵⁹Fe at a rate that was approximately 3 times that of the spleen and three-fourths that of the liver when ⁵⁹Fe uptake was related to organ weight at 24 hr postinjection. Autoradiography at the light microscopic level showed that ⁵⁹Fe was present in the lymphoid tumor cells and was not present in the interstitial, vascular, or necrotic areas of the tumor at either 24 or 72 hr after the injection of radioiron. The bone marrow, spleen, and bursa of Fabricius showed no increase in label above background. Tissue sections stained with Perls' reagent revealed little or no hemosiderin in the tumor or bone marrow but revealed moderate to marked increases in the amount of hemosiderin in the spleen and liver of most of the chickens that were inoculated with tumor. Lymphoid tumor-bearing chickens developed a progressive hypoferremia, and there was a precipitous drop in the packed cell volume. An increase occurred in both serum iron-binding capacity and transferrin levels over a 12-day period following tumor implantation. The uptake of iron by transplantable lymphoid tumor cells and an apparent blockage of iron reutilization were responsible for the rapid decline in serum iron.

¹ Published as Manuscript 914. Supported in part by General Research Support Grant 10-03-RR-208-014 (67) from the NIH.

² USPHS postdoctoral trainee in pathology.

Received 7/31/72. Accepted 10/27/72.

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