This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Cleaver, J. E. Search for Related Content PubMed PubMed Citation Articles by Cleaver, J. E.

DNA Repair with Purines and Pyrimidines in Radiation- and Carcinogen-damaged Normal and Xeroderma Pigmentosum Human Cells¹

Laboratory of Radiobiology, University of California, San Francisco, California 94122

Repair replication of DNA in cells exposed to UV, X-rays, or chemical carcinogens involves incorporation of both purine and pyrimidine precursors. Previous reports stating that purines are not involved in repair must be due to insufficient resolution in the reported experiments. Hypoxanthine was more efficiently incorporated by repair replication than other precursors such as adenine, deoxyadenosine, and guanine. Hydroxyurea did not inhibit hypoxanthine incorporation by repair replication. Cells from patients with the hereditary high-cancer disease xeroderma pigmentosum show reduced repair after exposure to UV, 4-nitroquinoline 1-oxide, and 1,3-bis(2-chloroethyl)-1-nitrosourea; they show normal repair after exposure to agents such as N-methyl-N'-nitro-N-nitrosoguanidine. These results allow agents to be classified according to whether or not xeroderma pigmentosum cells respond normally to them and illustrate the values and limitations of the use of xeroderma pigmentosum cells or measurements of DNA repair as test systems for carcinogens.

¹ Work performed under the auspices of the U. S. Atomic Energy Commission.

Received 8/21/72. Accepted 11/ 2/72.

This article has been cited by other articles:

				F. Ide, H. Oda, Y. Nakatsuru, K. Kusama, H. Sakashita, K. Tanaka, and T. Ishikawa Xeroderma pigmentosum group A gene action as a protection factor against 4-nitroquinoline 1-oxide-induced tongue carcinogenesis Carcinogenesis, April 1, 2001; 22(4): 567 - 572. [Abstract] [Full Text] [PDF]

				N. J. Bernheim and H. Falk Dr. Chemical, Physical, and Genetic Factors Interfering with DNA Repair-a Review International Journal of Toxicology, May 1, 1983; 2(3): 23 - 54. [Abstract] [PDF]

				J. H. ROBBINS, K. H. KRAEMER, M. A. LUTZNER, B. W. FESTOFF, and H. G. COON Xeroderma Pigmentosum: An Inherited Disease with Sun Sensitivity, Multiple Cutaneous Neoplasms, and Abnormal DNA Repair Ann Intern Med, February 1, 1974; 80(2): 221 - 248. [Abstract] [PDF]