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Some Effects of Chlorambucil on the Chromatin of Yoshida Ascites Sarcoma Cells¹

Department of Applied Biochemistry, Chester Beatty Research Institute, Institute of Cancer Research: Royal Cancer Hospital, Fulham Road, London, SW3 6JB, England

Some effects of chlorambucil (Leukeran) on the composition of the chromatin of Yoshida ascites sarcoma cells are described. Chlorambucil treatment of animals carrying a drug-sensitive line of the tumor results in the progressive removal of histone from the chromatin and its replacement by nonhistone protein present in the nucleus. The biosynthesis of histone is inhibited from the outset, while nonhistone protein biosynthesis declines 12 hr after treatment. These effects are related to morphological changes that occur in the nucleus.

¹ This work was supported by grants to the Chester Beatty Research Institute (Institute of Cancer Research: Royal Cancer Hospital) from the Medical Research Council and the Cancer Research Campaign.

Received 8/ 3/72. Accepted 11/ 3/72.