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Radioimmunoassay of Rat ₁-Fetoprotein¹

Department of Pathology, University of California at San Diego Medical School, La Jolla, California 92037

A competitive binding radioimmunoassay for rat ₁-fetoprotein (₁F) has been developed. This assay is approximately 20,000 times more sensitive than double diffusion in agar for ₁F detection, the threshold being approximately 5 ng. Further purification of apparently pure (by immunodiffusion and immunization) radiolabeled ₁F is required for the specific assay. An assay for a previously undetected contaminant(s) is used to check the purity of rat ₁F isolated by isoelectric focusing in order to obtain the purified unlabeled ₁F needed to establish the standard inhibition curve.

¹ Supported by Grant CA-12473 and Contract E-72-3258 from the National Cancer Institute, NIH, Bethesda, Md.

Received 12/ 1/72. Accepted 1/26/73.

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