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Bleomycin-induced Chromosomal Aberrations in Cultured Mammalian Cells¹

Children's Cancer Research Foundation [K. D. P., A. K.], and Department of Pathology [A. K.], Harvard Medical School, Boston, Massachusetts 02115

Mouse fibroblasts (L-929) and human cells (HeLa and CCRF-CEM lymphoblasts) exposed to bleomycin in vitro for 20 hr showed a marked inhibition in the number of cells entering mitosis. Bleomycin-induced chromosomal aberrations were seen in cells from the three cultured lines and were mostly in the form of chromatid gaps, breaks, and fragments, and multiradial translocations. Human lymphoblasts (CCRF-CEM) were more resistant to the bleomycin-induced mitotic inhibition and chromosomal aberrations than the L-929 or HeLa cells. A small number of chromatid fragments and translocations were also seen in cells exposed to bleomycin in the G₂ or post-DNA-synthetic part of the cell cycle.

¹ This study was supported in part by Research Grants C-6516 from the National Cancer Institute and FR-05526 from the Division of Research Facilities and Resources, NIH.

² To whom reprint requests should be sent, at Children's Cancer Research Foundation, 35 Binney St., Boston, Mass. 02115.

Received 8/16/72. Accepted 1/29/73.