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Isolation and Partial Characterization of Multiple DNA Polymerases of the Murine Myeloma, MOPC-21¹

Institute of Microbiology, Rutgers University, New Brunswick, New Jersey 08903

A method is presented for the isolation and initial purification of the DNA polymerases of the myeloma line MOPC-21. These activities are separable into two distinct fractions by diethylaminoethyl cellulose chromatography. Each fraction contains a DNA polymerase that is highly active on native calf thymus DNA that had been partially degraded with DNase ("activated" or "nicked" DNA) and another activity that transcribes ribopolymer strands in the presence of a complementary primer. Some distinctive properties of these enzymes are presented.

¹ Supported by Grant E-525A (NP-79B) from the American Cancer Society, National Science Foundation Grant GB-16871, NIH Grant GM-10395, and Damon Runyon Grant 1213. Preliminary reports of this work were presented at the 62nd and 63rd Annual Meetings of the American Society of Biological Chemists (June 1971, April 1972).

² Recipient of Postdoctoral Fellowship GM-46192 from the NIH.

³ Fellow of the New York City Cancer Research Institute Inc.

Received 7/24/72. Accepted 2/19/73.