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Effect of 5-Bromo-2-deoxyuridine on Transport of Deoxyglucose and Cycloleucine in 3T6 Fibroblasts¹

Department of Pathology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140

When 3T6 mouse fibroblasts in culture are grown in the presence of 5-bromo-2-deoxyuridine (50 µg/ml), they reach a saturation density which is only 50% of the saturation density reached by untreated control 3T6 cells. The uptake of deoxyglucose-³H and of cycloleucine-¹⁴C is decreased in cells grown in the presence of 5-bromo-2-deoxyuridine. The uptake of deoxyglucose-³H is also decreased in 3T6 mouse fibroblasts grown in the absence of 5-bromo-2-deoxyuridine, when they are incubated with the radioactive compound in the presence of 5-bromo-2-deoxyuridine, 50 µg/ml. These results confirm and extend previous observations reported in the literature on the effect of 5-bromo-2-deoxyuridine on the function of membranes in mammalian cells.

¹ This research was supported by Research Grant DRG-1019-C from the Damon Runyon Memorial Fund.

² Present address: National Institute of Radiological Sciences, Anagawa-4, Chiba-shi, Japan.

³ To whom reprint requests should be sent.

Received 11/13/72. Accepted 3/16/73.